It was Gatlin's second positive test, which under anti-doping rules could have resulted in a life ban.
US sprinter Justin Gatlin believes he is steadily catching up with Olympic and world champion Usain Bolt as the two head towards a likely showdown at the Rio de Janeiro Summer Games next year.
American Justin Gatlin felt the full force of the crowd's ire on Sunday and scowled back in response before finishing second to Usain Bolt in the Olympic 100metres final to miss his shot at becoming the oldest man to win the event at age 34.
Justin Gatlin won his first title since returning from a four-year doping ban with victory in the 60 metres at the world indoor championships on Saturday as the red-hot Americans grabbed four gold medals and a world record in Istanbul.
Justin Gatlin blasted out a warning that he is ready to challenge Usain Bolt's 100 metres hegemony when he stormed to a lifetime best 9.74 seconds to win the event in the opening Diamond League meeting of the season on Friday.
World 100 metres champion Justin Gatlin said on Tuesday he is "shocked and surprised" at doping allegations made against his coach Dennis Mitchell, like Gatlin a convicted doper, but added that he sacked him as soon as he heard about the issue.
Summary of sports events and persons who made news on Wednesday.
Olympic bronze medallist Justin Gatlin is not willing to call his surprising 100 metres victory over world record holder Usain Bolt a fluke.
In the history of sport, you were forced to ponder, had there ever been a champion more universally unloved than Gatlin, the twice doping offender who by winning world 100 metres gold on Saturday had ruined one of sport's great fairytale farewells.
Reaction to Gatlin's win and Bolt's surprise defeat:
Lauryn Williams clocked 10.93 seconds to join men's gold medallist Justin Gatlin as a world champion.
In-form American Justin Gatlin should beat Usain Bolt to win both the sprints at the World Athletics Championships but it would be foolhardy to write off the Jamaican, three-times world 100 metres World champion Maurice Greene said on Friday.
American Justin Gatlin stepped up his preparations for the Rio Olympics with an impressive victory in the 100 metres at the Diamond League in Shanghai on Saturday.
Gatlin stormed to the lead in the final 20 metres and defeated favoured youngster Christian Coleman in the 100 metres final at the US nationals/world championships trials on Friday.
Ethiopia's Almaz Ayana ran the second fastest women's 5,000 metres in history as she missed out on the world record by just over a second at the Rome Diamond League in Rome, on Thursday.
Justin Gatlin, the year's fastest at 100 and 200, had a bye in the 100 at the trials, but plans to challenge Usain Bolt at both distances in the August World Championships in Beijing.
A tired Usain Bolt and an emotionally fatigued Justin Gatlin progressed towards their second blockbuster sprint showdown of the World Athletics Championships by easing through the 200 metres heats in Beijing, on Tuesday.
Always the crown jewel in the Olympic athletics programme, the Rio 100 metres final is shaping up to be extraordinary for many reasons.
No spectators were present in the stadium, where the Games' opening and closing ceremonies will be held,
Organising committee says he was not kicked out
Sifan Hassan of the Netherlands broke the 23-year-old women's mile world record on Friday when she clocked four minutes 12.33 seconds in a race dedicated to former American runner Gabe Grunewald, who died from cancer last month at the age of 32.
'If you try to use every resource that you have, you won't feel like you've let yourself down. Don't think because wherever you came from that you cannot be successful. No matter if you make an Olympic team, you gave your heart and you put your heart out there to be as successful as you could be.'
Noah Lyles is the new Olympic 100m champion!
The coach of sprinter Justin Gatlin has stepped down as the head of the United States relay squads just months out from the Rio Olympics due to a change in USA Track and Field's criteria.
Usain Bolt says he is not yet ready for a duel with Justin Gatlin.
Factbox on American Justin Gatlin who won the 100 metres gold at the World Athletics Championships on Saturday at the age of 35.
The members of the US men's 4x100 metres relay team that won silver at the 2012 Olympics have been stripped of their medals following the doping conviction of sprinter Tyson Gay.
"So it's all about how I feel after Rio, if I feel I can really put my body through one more season. If I'm going to be focused and I'm going to be determined. That will determine if I compete after Rio. So we'll see how it goes."
Usain Bolt retained his 100 metres title at the IAAF World Championship, in Beijing, on Sunday.
Usain Bolt is eager to reassert his supremacy following an injury-plagued season in which Justin Gatlin took over.
Tyson Gay overcame Olympic bronze medallist Justin Gatlin's quicker start and sped to the year's fastest 100 metres, clocking 9.75 seconds at the US trials for the World championships on Friday.
Usain Bolt was made to look human by a combination of a Russian rainstorm and a fired-up Justin Gatlin on Sunday but the Jamaican superstar was still good enough to regain his world 100m title in a surging 9.77 seconds.
There is no one in the world like this Champion. As he bids farewell to sprinting, Laxmi Negi looks at the invaluable life lessons UB taught the world.